Identification of Proteus mirabilis Mutants with Increased Sensitivity to Antimicrobial Peptides

Identification of Proteus mirabilis mutants with increased sensitivity to antimicrobial peptides.

Antimicrobial peptides (APs) are important components of the innate defenses of animals, plants, and microorganisms. However, some bacterial pathogens are resistant to the action of APs. For example, Proteus mirabilis is highly resistant to the action of APs, such as polymyxin B (PM), protegrin, and the synthetic protegrin analog IB-367. To better understand this resistance, a transposon mutage...

Diversity of TEM mutants in Proteus mirabilis.

In a survey of resistance to amoxicillin among clinical isolates of Proteus mirabilis, 10 TEM-type beta-lactamases were characterized: (i) the well-known penicillinases TEM-1 and TEM-2, the extended-spectrum beta-lactamases (ESBLs) TEM-3 and TEM-24, and the inhibitor-resistant TEM (IRT) TEM-44 and (ii) five novel enzymes, a penicillinase TEM-57 similar to TEM-1, an ESBL TEM-66 similar to TEM-3,...

Proteus mirabilis ZapA metalloprotease degrades a broad spectrum of substrates, including antimicrobial peptides.

The 54-kDa extracellular metalloprotease ZapA is an important virulence factor of uropathogenic Proteus mirabilis. While ZapA has the ability to degrade host immunoglobulins (Igs), the dramatic attenuation of virulence in ZapA mutants suggests that this enzyme may have a broader spectrum of activity. This hypothesis was tested by in vitro assays with purified ZapA and an array of purified prote...

Transductional analysis of non-motile mutants in Proteus mirabilis.

Antimicrobial Susceptibility of Proteus mirabilis: Impact of Biofield Energy Treatment

Proteus mirabilis (P. mirabilis) is a species of Gram-negative and facultative anaerobic bacteria that shows swarming motility and urease activity. Proteus ranked third as the cause of hospital-acquired infections [1]. The organism is rod-shaped and motile bacterium with diverse mode of transmission [2]. P. mirabilis is a common causative organism of urinary tract infection (UTI) in the complic...